Gst fusion system manual

The Glutathione S-transferase (GST) Gene Fusion System is an integrated system for the expression, purification and detection of fusion proteins produced in E. coli. The system consists of three major components: pGEX plasmid vectors*, two GST Purification Modules and the GST Detection Module. A series of site-specific proteases complements the. GST-fusion protein purification. Glutathione is a tripeptide (Glu-Cys-Gly) that is the specific substrate for glutathione S-transferase (GST). When reduced glutathione is immobilized through its sulfhydryl group to a solid support, such as cross-linked beaded agarose, it can be used to capture pure GST or GST-tagged proteins via the enzyme-substrate binding reaction. Manual for GST fusion (v) for GE -SEGST -. kits www.doorway.ru Overview of the T7 expression system The T7 expression system is based on the use of the T7 bacteriophage promoter binding kinetics between glutathione and .

The Glutathione S-transferase (GST) Gene Fusion System is a versatile system for the expression, purification, and detection of fusion proteins produced in Eschericia coli. The system is based on inducible, high-level expression of genes or gene fragments as fusions with Schistosoma japonicum GST (1). Expression in E. coli yields fusion proteins with the. GST-fusion protein purification. Glutathione is a tripeptide (Glu-Cys-Gly) that is the specific substrate for glutathione S-transferase (GST). When reduced glutathione is immobilized through its sulfhydryl group to a solid support, such as cross-linked beaded agarose, it can be used to capture pure GST or GST-tagged proteins via the enzyme-substrate binding reaction. Numerous protocols for cloning and overexpression of soluble GST-fusion proteins are available in many molecular biology handbooks (5). Solubility of the GST-fusion protein is very important for successful GST pull-down and should be verified before attempting the pull-down experiment. To evaluate solubility, centrifuge a small volume of lysed cells.

S-transferase (GST) tagged proteins produced using the pGEX series of Consult the GST Gene Fusion System Handbook (ref. 1) for more. Protein expression in E. coli using GST-fusion systems is well with 50 µL GSH resin from various suppliers and purified per manufacturers' instructions. 1 may Abstract This unit describes the use of the glutathione-S-transferase (GST) gene fusion system as a method for high-level protein expression.